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Title   한국 여성의 자궁경부 조직에서 발견되는 인유두종 바이러스 16 형 E6 유전자 다형성 ( Polymorphisms in E6 Gene of Human Papillomavirus Type 16 Found in Cervical Tissues from Korean Women )
Publicationinfo   2000 Jan; 032(05): 875-884.
Key_word   Human papillomavirus, HPV 16, E6 gene, polymorphesm
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Abstract   Purpose: To examine the distribution of HPV 16 E6 polymorphisms and analyse the possible association between the polymorphisms and cervical cancer development in Korean women. Materials and Methods: Fifty-four cases of uterine cervical tissues containing HPV 16 DNA confirmed by polymerase chain reaction (PCR) from Korean women were subjected to investigate the E6 gene mutations. PCR-amplified products were sequenced by the fluorescent dideoxy termination method and the results obtained from sequencing were analysed. And newly designed PASA method was tried to develop rapid test for identification of the most commonly detected variation. Results: Among the 27 cervical cancer cases, only two (7.4%) was found as a prototype. Among 11 kind of variants identified in total, 4 variants (5 nucleotide sites) which were never reported before has been found, registered firstly to GenBank. The most frequently found variation was D25E, absolutely different from the previous reports from the western country. There was no statistically significant trend for the D25E variation to be more frequently detected in cancerous lesions than in noncancerous lesions. All of the DNA sequencing results observed could be confirmed by PASA method. Conclusion: These results suggest that Korean-specific genetic factors might operate during the cervical carcinogenesis.
저 자   김재원(Jae Weon Kim),노주원(Ju Won Roh),박노현(Noh Hyun Park),송용상(Yong Sang Song),강순범(Soon Beom Kang),이효표(Hyo Pyo Lee),김문홍(Moon Hong Kim)